Aptamers vs Antibodies

AptamersAntibodies

In vitro selection, identification and screening

In vivo selection, identification and screening


Ready useable to toxins, drugs and small molecules identification

Limited to identification of molecules that induce immune response

In vitro production by automated synthesis and PCR


More complicated production using animal host or bioreactor fermentation


Highly reproducible and pure product


Batch to batch variations in non-monoclonal preparations


Can function at different temperatures, pH and buffers 


Functionality usually limited to physiological conditions


Easily labelled with no loss of functionality


Labelling can cause loss of functionality


Denatured aptamers can be regenerated, aptamers are stable to long term storage and can be transported at ambient temperature

Antibodies have limited shelf life and are sensitive to temperature and may undergo denaturation